THC Levels of Cannabis Flowers at Mary Jane Berlin 2026 – A Cross-Sectional Analysis of Visitor Samples

Matthias Rohm, Patricia Moser, Christian Fuczik, Institut f. Hanfanalytik

Following the partial legalization of cannabis in Germany, interest in the actual potency of cannabis flowers has increased significantly. During Mary Jane Berlin 2026, 534 cannabis flower samples submitted by exhibition visitors were analyzed on-site using NIR spectroscopy.

1. Introduction

While THC levels are often displayed on packaging, shared on social media, or communicated through informal sources, there is limited data on the actual composition of the flowers used by consumers. Importantly, THC content is a key quality and safety parameter, as it substantially influences both the pharmacological effects and the risk profile of cannabis products.

With more than 70,000 visitors and several hundred exhibitors, Mary Jane Berlin 2026 brings together consumers, producers, retailers, and professionals from across the cannabis industry. This event provides a unique opportunity to collect a wide range of real consumer samples and investigate their CBD and THC contents.

2. Background

The analytical determination of tetrahydrocannabinol (THC) and cannabidiol (CBD) is essential for ensuring the quality control of cannabis products. Despite increasing market regulation, there are significant variations among cultivars, production batches, and countries of origin. Investigations conducted in our laboratory have revealed that actual THC concentrations often differ from the values declared on product labels.

3. Methods

On-site analyses were conducted using a portable near-infrared spectrometer (NIR). The NIR method used is based on a manufacturer-specific calibration model developed from an extensive reference database of chromatographically characterized cannabis samples. The limit of quantification for all cannabinoids was set at 1%.

To ensure a homogeneous sample matrix, the cannabis flowers were ground using a grinder. The homogenized material was transferred into an aluminum weighing dish and analyzed three times using NIR spectroscopy.

4. Materials Used

  • Portable NIR spectrometer (NIRLAB AG, Switzerland)
  • Webcam for photographic documentation
  • Stainless steel grinder, round, Ø 40 mm (NV Grinders, Austria)
  • Aluminum weighing dishes, round, Ø 57 mm (VWR International)
NIRLab NIR Spectrometer

Fig. 1: NIRLab NIR Spectrometer

Sample in weighing dish

Fig. 2: Sample in an aluminum weighing dish

5. Results

A total of 534 samples were submitted for analysis. These were categorized as:

  • 13 CBD cultivars (CBD > 1% and THC < 1%)
  • 5 CBD–THC hybrid cultivars (CBD > 1% and THC > 1%)
  • 516 THC cultivars (CBD < 1% and THC > 1%)

The THC cultivars demonstrated a median THC concentration of 17.4%, with values ranging from 5.9% to 25.7% THC. These samples fall within the range of modern, high-potency cannabis flowers increasingly observed across Europe.

Table 1: Results hybrid cultivars

Table 1: Results hybrid cultivars

Distribution of THC concentrations

Fig. 3: Distribution of THC concentrations in submitted samples

Analysis result – NIR spectrum

Fig. 4: Analysis result – NIR spectrum

Analysis result – Server access

Fig. 5: Analysis result – Accessible via hanfanalytik.at server

6. Discussion

The median THC concentration of 17.4% identified in this study is higher than the historical European averages, reflecting the continuing trend toward more potent cannabis products. Importantly, none of the samples analyzed had THC concentrations above 30%.

7. Summary

  • 534 cannabis flower samples analyzed
  • On-site analysis using NIR spectroscopy
  • Median THC concentration: 17.4%
  • THC range: 5.9–25.7%

References

[1] Mary Jane Berlin – maryjane-berlin.com
[2] Rafiq et al. (2024). Plants, 13(6), 833.
[3] Sánchez-Carnerero et al. (2025). Frontiers in Plant Science, 16.
[4] European Union Drugs Agency (2026). European Drug Report 2026.